Analysis of the Bacterial Community of Metal Scrap Using an Enrichment Culture Approach

Microbiologically influenced corrosion (MIC) of metal alloys is promoted by biofilms formed on surfaces. In the marine environment, MIC causes serious infrastructure problems, which lead to significant economic losses. this study, we used an enrichment culture approach examine bacterial community that grows surface at levels below detection limit as a preliminary study for developing guidelines prevent biofilm formation. An was employed analyze without and corrosion. Genomic DNA extracted from sample after incubation in with piece, then V3–V4 variable regions 16S rRNA gene were amplified using genomic template. Subsequently, next-generation sequencing approach, sequenced, analyzed QIIME 2 microbiome bioinformatics platform. Using more than 80 genera detected Sphingomonas bacteria exhibiting highest relative abundance (44%). These results demonstrated method could be useful analysis limits, will serve basis development guidelines.